Browsing by Author "Salih,Sakeena NourEldine"

Now showing 1 - 2 of 2

Association of expression C/C .G/G, C/G Genotypes with Glycated hemoglobin (HbA1c) levels in Sudanese children with type 1 Diabetes
(Sud Med Lab J, 2023) Hussein,Hiba Omer Abdelrahman; Abdalla,Sababil Salih; Salih,Sakeena NourEldine; Alkhidir,Abdulazeem Abdulsalam Ibrahim; Mahdi,Mohamed Abdelgadir
Background: Type 1 diabetes (T1D) is a common complex metabolic disease characterized by the autoimmune destruction of insulin-producing B cells of the pancreatic islets. Due to a drastic loss of B cells, resulting in no to lowinsulin production,. Genetic factors play a significant role in the aetiology of T1D, However, the main causes of morbidity and mortality today are the complications that arise from even mild hyperglycaemia in T1D cases. Glycemic control is often measured using an assay called haemoglobin A1C (HbA1c), that provides an estimate of average blood glucose in individuals over a 3month period. Aim of the study to correlate between the frequencies of C/C,G/G and C/G genotypes and HBA1C levels in T1D patients. Objective: To evaluate the significant correlation between frequencies of genotypes and HBA1C levels. Methods: A total of 100 Sudanese subjects with T1D were enrolled in this study, on the average age between 5 to 18 years. The study was conducted in diabetes central hospitals in Khartoum state. In order to determine the HLA gene polymorphism, the allele-specific-refractory mutation system-polymerase chain reaction (ARMS-PCR) method was utilized. Results: There were no significant correlation (r= 0.074, P-value = 0.497) between frequencies of genotypes and HBA1C levels. Conclusion: This study identified that there’s no significant correlation between frequencies of genotypes and HBA1C levels.
The Association of HLA-DRB1/DQB1 Genes with Type 1 Diabetes Mellitus among Sudanese Children and Adolescents
(Al-Kindy College Medical Journal, 2023) Hussein,Hiba Omer AbdelRhman; Abdalla,Sababi Salih; Salih,Sakeena NourEldine; Abdrabo,AbdElkarim Abobaker; Mahdi,Mohamed Abdelgadir
Objective: This study was conducted to identify the association of HLA-DRB1/DQB1 genes with the susceptibility or resistance to type 1 diabetes mellitus (T1D) among patients between the ages of five and eighteen. Subjects and Methods: The study included 200 Sudanese participants, ages ranging from 5 to 18. One hundred participants were healthy non-diabetic as the control group and 100 with T1D as the case group. The investigation was carried out in Khartoum state. The selection of patients with T1D was from diabetic centers and hospitals. The allele-specific-refractory mutation system-polymerase chain reaction (ARMS-PCR) technique was applied to identify the HLA gene polymorphism. Results: There was a significant difference in genotype frequency across the groups in the current investigation (Kruskal-Wallis, p-value= 0.021). Whereas CG was not substantially different across groups (Chi-square, p-value=0.116), the CC genotype was considerably greater (46.0%) in patients (Chi-square Adjusted p-value0.001). Conclusion: This study found that patients' genotypes and allele frequencies are significantly correlated when compared to those of healthy participants.