Hashim, Nada TawfigFathima, SadiahHisham, Nurain MohammadShivappa, PoojaMagaogao, Michael V.Islam, Md SofiqulAhmed, Sara FaisalBabiker, RashaRahman, MuhammedMustahsen2025-10-102024https://dspace.nu.edu.sd/handle/nusu/99Periodontal disease, characterized by bacterial plaque accumulation and subsequent im muneresponse, can lead to gingivitis and periodontitis if untreated. Salivary alpha-amylase (sAA) has emerged as a potential biomarker with implications in periodontal disease progression. Objec tives: This study aimed to assess and compare salivary alpha-amylase levels in individuals with periodontitis and healthy controls and to investigate its relationship with clinical parameters of periodontal disease. Forty-five participants were categorized into periodontally healthy (n = 13), Stage I and II Periodontitis (n = 17), and Stage III and IV periodontitis (n = 15) groups. Saliva samples were collected and analyzed using ELISA kits. Statistical analyses included tests for normality, group comparisons, post hoc analysis, and correlation analysis. Significant differences in salivary alpha-amylase levels were observed among severity groups (p < 0.05), with higher levels in periodon titis patients than healthy controls. Spearman correlation revealed moderate positive associations between alpha-amylase levels and probing depth (PD) and clinical attachment loss (CAL). Elevated salivary alpha-amylase levels were found to be associated with more severe periodontal disease, suggesting its potential as a biomarker for periodontitis severity. These findings support the utility of salivary biomarkers in periodontal disease diagnosis and monitoring, although further validation and standardization are warranted for clinical application.enperiodontal diseasesalivary alpha-amylasebiomarkersperiodontitissaliva diagnosticsclinical attachment lossprobing depthArticle